Expression and purification of chicken beta interferon and its antivirus immunological activity
M Cai, F Zhu, P Shen - Protein Expression and Purification, 2012 - Elsevier
Interferon beta (IFN-β) belongs to a class of natural proteins that can inhibit virus replication.
In this paper, nucleic acid sequence encoding chicken interferon beta (chIFN-β) mature
protein was cloned and highly expressed in Escherichia coli (E. coli) by 1mM IPTG
induction. The expressed chIFN-β was about 20% of total bacterial protein. The recombinant
protein in form of inclusion body was then solubilised, refolded and purified to a purity of
greater than 95% by immobilized metal ion affinity chromatography (IMAC). This …
In this paper, nucleic acid sequence encoding chicken interferon beta (chIFN-β) mature
protein was cloned and highly expressed in Escherichia coli (E. coli) by 1mM IPTG
induction. The expressed chIFN-β was about 20% of total bacterial protein. The recombinant
protein in form of inclusion body was then solubilised, refolded and purified to a purity of
greater than 95% by immobilized metal ion affinity chromatography (IMAC). This …
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